Cell proliferation is the process of cell replication to increase the number of cells. Cell proliferation plays a vital role in cellular and tissue homeostasis for proper development, growth and maintenance of human beings. In case of few disease conditions, the cell proliferation rate is either increased or decreased. Hence, cell proliferation assays have become an integral part of the clinical diagnosis of various disease indications. Cell proliferation assays are used for investigational studies of cell activation, assessing cytotoxicity & clinically, as well as to test the effect of pharmacological interventions & growth factors on human cells. Thus, cell proliferation assays are used to measure cell count, the percentage change in cell proportion and division rate of the cell.